Detection of endotoxin in commercial L-asparaginase preparations by complement fixation and separation by chromatography.

نویسندگان

  • M Loos
  • S Vadlamudi
  • M Meltzer
  • S Shifrin
  • T Borsos
  • A Goldin
چکیده

Of 15 L-asparaginase preparations tested, 13 inhibited whole human serum complement; the inhibitory effect ranged from 12 to 45%. We have partially characterized the anticomplementary activity in one of the L-asparaginase preparations. The anticomplementary activity was separable from the L-asparaginase activity on Sephadex G-200. Fractions containing L-asparaginase had no effect either on whole human serum complement or on human serum Cl, the first component of complement; these fractions were therapeutic in leukemic mice. Fractions containing the anticomplementary activity had no significant therapeutic effect in leukemic mice. L-asparaginase and the anticomplementary factor are antigenically distinct, and the anticomplementary factor was shown to be antigenically related to Escherichia coli lipopolysaccharide (endotoxin).

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عنوان ژورنال:
  • Cancer research

دوره 32 11  شماره 

صفحات  -

تاریخ انتشار 1972